[Metabonomic study on protective effect of xiaoyao powder for acute hepatic injury in rats].

ABSTRACT

OBJECTIVE: To study the metabonomic for acute hepatic injury in rats and the hepato-protective effect of Xiaoyao Powder in rats. METHODS: Male rats were treated with Xiaoyao Powder decoction through intragastric for 7 days. 0.2% CCl4 peanut oil solution were given by intraperitoneal injection in one hour after 7 days. The rats were fasted for 16 hours, but water was given. Hepatic tissues were used for histopathological examination. Pre-column derivatization-GC-MS was used to detect the changes in rats serum metabolites. Mass spectrometry analysis, PCA and other technolgy technical were used to analyze the differences among their metabolites. RESULTS: Compared with normal group, model group rats hepatic tissue was destroyed obviously and nodular regenerative hyperplasia of hepatic cells were clear. Alanine, glycine, serine, threonine, aminomalonic acid, malic acid, aspartic acid, ornithine, lysine, inositol and glutamine were significantly increased in serum. All the indicators were increased after treated with Xiaoyao Powder. CONCLUSION: Xiaoyao Powder has curative effect on acute hepatic injury, and its mechanism may be related to adjusting aliphatic acid metabolism and promoting amino acids generated function.