Inhibition of the invasion and migration of renal carcinoma 786osi3 cells in vitro and in vivo by Koelreuteria formosana extract.
Mol Med Rep
; 10(6): 3334-42, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25241754
ABSTRACT
Koelreuteria formosana ethanolic extract (KFEE) is obtained from natural plants that are endemic to Taiwan. In a previous study, it was demonstrated that KFEE inhibited low-density lipoprotein (LDL) and prevented oxidized LDLinduced apoptosis in endothelial cells. In the present study, KFEE was shown to inhibit the invasion and migration of 786OSI3 renal cell carcinoma (RCC) cells while not exhibiting any cytotoxic effects. 786OSI3 cells were treated with KFEE at numerous concentrations of ≤100 µg/ml for 24 h. In order to examine the effects of KFEE, cells were then subjected to a series of assays for cell viability (MTT), wound healing migration, cell invasion and migration, gelatin zymography, casein zymography and immunofluorescence, as well as western blot analysis. KFEE was shown to decrease levels of matrix metalloproteinase2, phosphorylated (p)focal adhesion kinase Try925, ppaxillin Ser178, pmitogenactivated protein kinase kinase 1/2, pmyosin light chain and pextracellular signal-regulated kinase 1/2 in 786-0-SI3 cells. Reduction of lung metastases was observed in KFEE-treated mice compared with vehicletreated control mice. KFEE inhibited the invasion of RCC cells and may have the potential for use as a chemopreventive agent against RCC metastasis.
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Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Carcinoma de Células Renais
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Movimento Celular
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Neoplasias Renais
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Invasividade Neoplásica
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Antineoplásicos
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2014
Tipo de documento:
Article