Bio-mimetic nanostructure self-assembled from Au@Ag heterogeneous nanorods and phage fusion proteins for targeted tumor optical detection and photothermal therapy.
Sci Rep
; 4: 6808, 2014 Oct 28.
Article
em En
| MEDLINE
| ID: mdl-25348392
ABSTRACT
Nanomaterials with near-infrared (NIR) absorption have been widely studied in cancer detection and photothermal therapy (PTT), while it remains a great challenge in targeting tumor efficiently with minimal side effects. Herein we report a novel multifunctional phage-mimetic nanostructure, which was prepared by layer-by-layer self-assembly of Au@Ag heterogenous nanorods (NRs) with rhodamine 6G, and specific pVIII fusion proteins. Au@Ag NRs, first being applied for PTT, exhibited excellent stability, cost-effectivity, biocompatibility and tunable NIR absorption. The fusion proteins were isolated from phage DDAGNRQP specifically selected from f8/8 landscape phage library against colorectal cancer cells in a high-throughput way. Considering the definite charge distribution and low molecular weight, phage fusion proteins were assembled on the negatively charged NR core by electrostatic interactions, exposing the N-terminus fused with DDAGNRQP peptide on the surface. The fluorescent images showed that assembled phage fusion proteins can direct the nanostructure into cancer cells. The nanostructure was more efficient than gold nanorods and silver nanotriangle-based photothermal agents and was capable of specifically ablating SW620 cells after 10â
min illumination with an 808â
nm laser in the light intensity of 4â
W/cm(2). The prepared nanostructure would become an ideal reagent for simutaneously targeted optical imaging and PTT of tumor.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fototerapia
/
Neoplasias Colorretais
/
Proteínas do Capsídeo
/
Biomimética
/
Nanoestruturas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China