Transport Function of Rice Amino Acid Permeases (AAPs).
Plant Cell Physiol
; 56(7): 1355-63, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25907566
ABSTRACT
The transport function of four rice (Oryza sativa) amino acid permeases (AAPs), OsAAP1 (Os07g04180), OsAAP3 (Os06g36180), OsAAP7 (Os05g34980) and OsAAP16 (Os12g08090), was analyzed by expression in Xenopus laevis oocytes and electrophysiology. OsAAP1, OsAAP7 and OsAAP16 functioned, similarly to Arabidopsis AAPs, as general amino acid permeases. OsAAP3 had a distinct substrate specificity compared with other rice or Arabidopsis AAPs. OsAAP3 transported the basic amino acids lysine and arginine well but selected against aromatic amino acids. The transport of basic amino acids was further analyzed for OsAAP1 and OsAAP3, and the results support the transport of both neutral and positively charged forms of basic amino acids by the rice AAPs. Cellular localization using the tandem enhanced green fluorescent protein (EGFP)-red fluorescent protein (RFP) reporter pHusion showed that OsAAP1 and OsAAP3 localized to the plasma membrane after transient expression in onion epidermal cells or stable expression in Arabidopsis.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Oryza
/
Regulação Enzimológica da Expressão Gênica
/
Regulação da Expressão Gênica de Plantas
/
Sistemas de Transporte de Aminoácidos
Idioma:
En
Revista:
Plant Cell Physiol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos