High dose of epigallocatechin-3-gallate inhibits proliferation and induces apoptosis of H9C2 cardiomyocytes through down-regulation of SIRT1.
Pharmazie
; 70(1): 12-6, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25975092
ABSTRACT
BACKGROUND:
Previous studies have suggested that high doses of (-)-epigallocatechin-3-gallate (EGCG) can induce toxicity in the liver, kidneys, and intestine. However, there have been no reports of myocardiotoxicity following treatment with EGCG. In this study, we investiged the proliferation and apoptosis of H9C2 cardiomyocytes treated with high dose of EGCG.METHODS:
Cell proliferation was measured by CCK8 assay, cell apoptosis rate was evaluated by TUNEL assay, and the expression alterations of Sirtuin 1 (SIRT1) protein was detected by Western blotting.RESULTS:
EGCG inhibits proliferation and induces apoptosis in time- and dose-dependent manner in H9C2 cardiomyocytes. SIRT1 participates in the inhibitory effect of EGCG on cell proliferation and apoptosis induction in H9C2 cardiomyocytes.CONCLUSION:
This study demonstrates that high doses of EGCG inhibit proliferation and induce apoptosis in H9C2 cardiomyocytes. Down-regulation of SIRT 1 protein expression may be involved.
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Base de dados:
MEDLINE
Assunto principal:
Catequina
/
Apoptose
/
Miócitos Cardíacos
/
Proliferação de Células
/
Sirtuína 1
/
Antioxidantes
Idioma:
En
Revista:
Pharmazie
Ano de publicação:
2015
Tipo de documento:
Article