Simultaneous determination and pharmacokinetic study of Atractylenolide I, II and III in rat plasma after intragastric administration of Baizhufuling extract and Atractylodis extract by UPLC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci
; 993-994: 86-92, 2015 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-26001909
ABSTRACT
A simple and rapid ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of Atractylenolide I, II and III in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate, using schisandrin as internal standard (IS). Chromatographic separation was accomplished on a Thermo Hypersil GOLD C18 column (2.1mm×50mm, 1.9µm) with mobile phase consisting of acetonitrile and 0.1% formic acid-water (5050, v/v). The detection was carried out by ESI-MS (positive ionization mode) and low-energy collision dissociation tandem mass spectrometric analyses using the multiple-reaction monitoring (MRM) scan mode. The quantification was performed using the transitions of the protonated moleculeâproduct ion at m/z 231.0â185.1 for Atractylenolide I, at m/z 233.1â187.1 for Atractylenolide II and at m/z 249.1â231.1 for Atractylenolide III, respectively. Method validation revealed excellent linearity over investigated range together with satisfactory intra- and inter-day precision, accuracy, matrix effects and extraction recoveries. This method was successfully applied to the comparative pharmacokinetic study of Atractylenolide I, II and III in rat plasma after intragastric administration of Baizhufuling extract and Atractylodis extract.
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Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Sesquiterpenos
/
Medicamentos de Ervas Chinesas
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Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas em Tandem
/
Lactonas
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China