Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library.
Mol Med Rep
; 12(5): 7051-8, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26323695
ABSTRACT
Accumulating evidence has suggested that fibroblast growth factor 3 (FGF3) is expressed in breast cancer and correlates with the stage and grade of the disease. In the present study, a specific FGF3binding peptide (VLWLKNR, termed FP16) was isolated from a phage display heptapeptide library with FGF3. The peptide FP16 contained four identical (WLKN) amino acids and demonstrated high homology to the peptides of the 188194 (TMRWLKN) site of the highaffinity FGF3 receptor fibroblast growth factor receptor 2. Functional analyses indicated that FP16 mediated significant inhibition of FGF3induced cell proliferation, arrested the cell cycle at the G0/G1 phase by increasing proliferationassociated protein 2G4, suppressing cyclin D1 and proliferating cell nuclear antigen, and inhibited the FGF3induced activation of extracellular signalregulated kinase 1/2 and Akt kinase. Taken together, these results demonstrated that the peptide FP16, acting as an FGF3 antagonist, is a promising therapeutic agent for the treatment of breast cancer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Fator 3 de Crescimento de Fibroblastos
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2015
Tipo de documento:
Article