Rapid effects of essential fatty acid deficiency on growth and development parameters and transcription of key fatty acid metabolism genes in juvenile barramundi (Lates calcarifer).
Br J Nutr
; 114(11): 1784-96, 2015 Dec 14.
Article
em En
| MEDLINE
| ID: mdl-26411329
ABSTRACT
Barramundi (Lates calcarifer), a catadromous teleost of significant and growing commercial importance, are reported to have limited fatty acid bioconversion capability and therefore require preformed long-chain PUFA (LC-PUFA) as dietary essential fatty acid (EFA). In this study, the response of juvenile barramundi (47·0 g/fish initial weight) fed isolipidic and isoenergetic diets with 8·2% added oil was tested. The experimental test diets were either devoid of fish oil (FO), and thus with no n-3 LC-PUFA (FO FREE diet), or with a low inclusion of FO (FO LOW diet). These were compared against a control diet containing only FO (FO CTRL diet) as the added lipid source, over an 8-week period. Interim samples and measurements were taken fortnightly during the trial in order to define the aetiology of the onset and progression of EFA deficiency. After 2 weeks, the fish fed the FO FREE and FO LOW diets had significantly lower live-weights, and after 8 weeks significant differences were detected for all performance parameters. The fish fed the FO FREE diet also had a significantly higher incidence of external abnormalities. The transcription of several genes involved in fatty acid metabolism was affected after 2 weeks of feeding, showing a rapid nutritional regulation. This experiment documents the aetiology of the onset and the progression of EFA deficiency in juvenile barramundi and demonstrates that such deficiencies can be detected within 2 weeks in juvenile fish.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Essenciais
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Regulação Enzimológica da Expressão Gênica
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Deficiências Nutricionais
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Dieta
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Doenças dos Peixes
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Peixes
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Fígado
País/Região como assunto:
Oceania
Idioma:
En
Revista:
Br J Nutr
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Austrália