The in vitro effect of 1 alpha,25-dihydroxyvitamin D3 on insulin production by neonatal rat islets.
Biochem Biophys Res Commun
; 164(1): 413-8, 1989 Oct 16.
Article
em En
| MEDLINE
| ID: mdl-2679556
ABSTRACT
The in vitro effect of 1 alpha,25-dihydroxyvitamin D3 on the function of beta cells of the endocrine pancreas was investigated. Neonatal islets maintained in serum-free medium, or medium supplemented with 0.5% fetal bovine serum achieved a 2.5-fold increase in medium insulin levels in response to 10(8) M 1 alpha,25-dihydroxyvitamin D3 (P less than 0.001). The effect of 1,25-dihydroxyvitamin D3 required at least 96 h treatment to become evident and was similar at medium glucose concentrations of 10 and 20 mM. Cell-associated insulin was increased in 1 alpha,25-dihydroxyvitamin D3-treated cultures maintained in 0.5% serum. These data suggest that 1 alpha,25-dihydroxyvitamin D3 may have a direct effect in the beta cell.
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Base de dados:
MEDLINE
Assunto principal:
Calcitriol
/
Ilhotas Pancreáticas
/
Insulina
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Austrália