Effects of dietary supplementation with epidermal growth factor-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets.
Br J Nutr
; 115(9): 1509-20, 2016 05.
Article
em En
| MEDLINE
| ID: mdl-26983845
The aim of the present study was to assess the effects of dietary supplementation with epidermal growth factor (EGF)-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets. In total, forty piglets weaned at 21-26 d of age were assigned to one of the five groups that were provided basic diet (control group) or diet supplemented with S. cerevisiae expressing either empty-vector (INVSc1(EV) group), tagged EGF (T-EGF) (INVSc1-TE(-) group), extracellular EGF (EE-EGF) (INVSc1-EE(+) group) or intracellular EGF (IE-EGF) (INVSc1-IE(+) group). All treatments were delivered as 60·00 µg/kg body weight EGF/d. On 0, 7, 14 and 21 d, eight piglets per treatment were sacrificed to analyse the morphology, activities and mRNA expressions of digestive enzymes, as well as Ig levels (IgA, IgM, IgG) in duodenal mucosa. The results showed significant improvement on 7, 14 and 21 d, with respect to average daily gain (P<0·05), mucosa morphology (villus height and crypt depth) (P<0·05), Ig levels (P<0·01), activities and mRNA expressions of digestive enzymes (creatine kinase, alkaline phosphatase, lactate dehydrogenase and sucrase) (P<0·05) and the mRNA expression of EGF-receptor (P<0·01) in NVSc1-TE(-), INVSc1-EE(+) and INVSc1-IE(+) groups compared with control and INVSc1(EV) groups. In addition, a trend was observed in which the INVSc1-IE(+) group showed an improvement in Ig levels (0·05
Assuntos
Palavras-chave
ALP alkaline phosphatase; Administration forms of epidermal growth factor; BW body weight; CK creatine kinase; Duodenal development; EE-EGF extracellular expressing epidermal growth factor; EGF epidermal growth factor; EGF-R epidermal growth factor receptor; GIT gastrointestinal tract; IE-EGF intracellular expressing epidermal growth factor; INVSc1(EV) empty vector-expressing-Saccharomyces cerevisiae; INVSc1-EE(+) EE-EGF-expressing-Saccharomyces cerevisiae; INVSc1-IE(+) IE-EGF-expressing-Saccharomyces cerevisiae; INVSc1-TE(−) T-EGF protein-expressing-Saccharomyces cerevisiae; LDH lactate dehydrogenase; T-EGF tagged epidermal growth factor; Weaned piglets; Weaning stress
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Suplementos Nutricionais
/
Duodeno
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Fator de Crescimento Epidérmico
/
Mucosa Intestinal
Idioma:
En
Revista:
Br J Nutr
Ano de publicação:
2016
Tipo de documento:
Article
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Suplementos Nutricionais
/
Duodeno
/
Fator de Crescimento Epidérmico
/
Mucosa Intestinal
Idioma:
En
Revista:
Br J Nutr
Ano de publicação:
2016
Tipo de documento:
Article