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Deconvoluting the Dual Antiplatelet Activity of a Plant Extract.
Kontogianni, Vassiliki G; Tsoumani, Maria E; Kellici, Tahsin F; Mavromoustakos, Thomas; Gerothanassis, Ioannis P; Tselepis, Alexandros D; Tzakos, Andreas G.
Afiliação
  • Kontogianni VG; Laboratory of Organic Chemistry, Department of Chemistry, University of Ioannina , 45110 Ioannina, Greece.
  • Tsoumani ME; Atherothrombosis Research Centre/Laboratory of Biochemistry, Department of Chemistry, University of Ioannina , 45110 Ioannina, Greece.
  • Kellici TF; Laboratory of Organic Chemistry, Department of Chemistry, University of Ioannina , 45110 Ioannina, Greece.
  • Mavromoustakos T; Laboratory of Organic Chemistry, Department of Chemistry, National and Kapodistrian University of Athens , Athens, Greece.
  • Gerothanassis IP; Laboratory of Organic Chemistry, Department of Chemistry, National and Kapodistrian University of Athens , Athens, Greece.
  • Tselepis AD; Laboratory of Organic Chemistry, Department of Chemistry, University of Ioannina , 45110 Ioannina, Greece.
  • Tzakos AG; Atherothrombosis Research Centre/Laboratory of Biochemistry, Department of Chemistry, University of Ioannina , 45110 Ioannina, Greece.
J Agric Food Chem ; 64(22): 4511-21, 2016 Jun 08.
Article em En | MEDLINE | ID: mdl-27161160
A thorough evaluation of the antiplatelet activity profile of hexane olive leaf extract in human platelets indicated a potent activity accomplished through a two axis inhibition of platelet activation triggered both by ADP and thrombin. To delineate the extract components responsible for this dual activity, an NMR based method was established to determine and quantify the triterpenoid content leading to the characterization of uvaol, erythrodiol, and oleanolic acid. The antiplatelet profile of the total extract and of the 3 determined triterpenoids was evaluated against in vitro platelet aggregation induced by several platelet agonists as also on PAC-1 binding and P-selectin membrane expression both in healthy volunteers and in platelets from patients with an acute coronary syndrome receiving dual antiplatelet therapy with aspirin and ticagrelor. The extract was identified to inhibit ADP-induced platelet activation due to its erythrodiol content and TRAP-induced platelet activation due to the activity of uvaol and oleanolic acid.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Inibidores da Agregação Plaquetária / Extratos Vegetais / Agregação Plaquetária / Olea Idioma: En Revista: J Agric Food Chem Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Grécia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Inibidores da Agregação Plaquetária / Extratos Vegetais / Agregação Plaquetária / Olea Idioma: En Revista: J Agric Food Chem Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Grécia