Fluorescent Detection of Lipid Droplets and Associated Proteins.
Curr Protoc Cell Biol
; 71: 4.31.1-4.31.14, 2016 06 01.
Article
em En
| MEDLINE
| ID: mdl-27245427
ABSTRACT
Excess lipid is stored in intracellular organelles known as lipid droplets. This unit discusses techniques for the visualization of lipid droplets and associated proteins in cultured mammalian cells. Protocols for the detection of lipid droplets in fixed or live cells with BODIPY 493/503 are included. The best method for combining visualization of intracellular lipid droplets with indirect immunofluorescent detection of lipid droplet-associated proteins is described. Techniques for sample fixation and permeabilization must be chosen carefully to avoid alterations to lipid droplet morphology. Immunofluorescent detection of perilipin 2, a broadly expressed, lipid droplet-associated protein, widely used as a marker for lipid droplet accumulation, is presented as an example. Finally, a simple protocol for enhancing lipid droplet accumulation through supplementation with excess fatty acid is included. © 2016 by John Wiley & Sons, Inc.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunofluorescência
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Gotículas Lipídicas
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Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
Curr Protoc Cell Biol
Ano de publicação:
2016
Tipo de documento:
Article