Diurnal Variation of the Peripheral Cholinergic Antiinflammatory Function in Mice.
CNS Neurosci Ther
; 22(9): 764-70, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27306582
ABSTRACT
AIMS:
Cholinergic antiinflammatory (CAI) pathway functions importantly in inflammation via α7 nicotinic acetylcholine receptors (α7nAChR). The present work tested circadian rhythm in peripheral CAI activity and validities of CAI activity and glucocorticoids in chronotherapy for lipopolysaccharide (LPS)-induced shock.METHODS:
Vesicular acetylcholine transporter (VAChT) expressed in liver and kidney was examined every 3 h in C57BL/6 mice. Proinflammatory cytokines in serum and survival time in shock were monitored after LPS injection every 3 h. Mifepristone, antagonist of glucocorticoid receptors, and methyllycaconitine (MLA), antagonist of α7nAChR, were administrated before LPS to block antiinflammatory function of endogenous glucocorticoids and acetylcholine.RESULTS:
Both levels of tumor necrosis factor α, interleukin 1ß, and interleukin 6 and mortality exhibited diurnal variations with prominent peaks when LPS was given at 1500, and the minimum mortality occurred at 0000. Expression of VAChT increased during resting period. MLA increased serum proinflammatory cytokines slightly, but not affected survival rate. Both differences in cytokines and in survival times between LPS injection at 1500 and 0000 were eliminated by mifepristone, but not by MLA.CONCLUSION:
Peripheral CAI pathway exerts more powerful antiinflammatory effect during resting period. Glucocorticoids appear to be efficient in chronotherapy for septic shock.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetilcolina
/
Citocinas
/
Ritmo Circadiano
/
Proteínas Vesiculares de Transporte de Acetilcolina
/
Inflamação
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
CNS Neurosci Ther
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China