Bonny light crude oil-induced alteration in levels of testicular stress proteins is accompanied by apoptosis in rats after treatment withdrawal.
J Basic Clin Physiol Pharmacol
; 28(2): 123-131, 2017 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-27861138
ABSTRACT
BACKGROUND:
The folkloric use of Bonny light crude oil (BLCO) in the treatment of gastrointestinal disorders and as an anti-poison is a generally acceptable practice in the Niger Delta area of Nigeria. The testicular dysfunction induced by BLCO exposure is of public concern with a view to its folkloric usage. The present study investigated the effects of BLCO exposure and withdrawal on the levels of testicular stress proteins and apoptosis-related proteins in rats.METHODS:
Adult male Wistar rats were exposed to 800 mg/kg body weight of BLCO for 7 days. One-half of the rats in each group were sacrificed on day 8, while the remaining one-half stayed an additional 45 days without treatment.RESULTS:
Western blot analysis showed that administration of BLCO resulted in a significant increase in the levels of stress proteins and apoptosis-related proteins by 50% and above relative to control, except cytosolic nuclear factor-κB (NF-κB), which decreased significantly relative to control. This was followed by a concomitant increase in the expression of caspase-3, FasL, and NF-κB by immunofluorescence staining within the testicular germ cells. Apoptosis showed a significant increase in TUNEL-positive cells. Following withdrawal of treatment, BLCO-mediated alteration in stress proteins and induction of apoptosis persisted relative to control.CONCLUSIONS:
Collectively, BLCO induced irreversible alteration in testicular stress proteins and apoptosis in rats within the time course of investigation. These findings highlight the potential long-term adverse effects of BLCO on individuals unduly exposed to BLCO.
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Base de dados:
MEDLINE
Assunto principal:
Síndrome de Abstinência a Substâncias
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Testículo
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Petróleo
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Apoptose
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Mediadores da Inflamação
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Proteínas de Choque Térmico
Idioma:
En
Revista:
J Basic Clin Physiol Pharmacol
Ano de publicação:
2017
Tipo de documento:
Article