Matrix Solid-Phase Dispersion Coupled with High-Performance Liquid Chromatography Diode Array Detection for Simultaneous Determination of Four Lipophilic Constituents from Salvia miltiorrhiza Bunge.
J Chromatogr Sci
; 55(3): 316-326, 2017 03 01.
Article
em En
| MEDLINE
| ID: mdl-27903553
ABSTRACT
A simple, rapid and efficient method based on matrix solid-phase dispersion coupled with high-performance liquid chromatography was developed for determination of lipophilic constituents, including dihydrotanshinone, tanshinone I, cryptotanshinone and tanshinone II A in Salvia miltiorrhiza Bunge Box-Behnken design was employed for optimization of the extraction conditions of matrix solid-phase dispersion, including mass ratio of dispersant to sample, volume of elution solvent, and amount of cleanup reagent. The optimal experimental results were obtained using 0.27 g of acid alumina as dispersant, 13 mL of acetonitrile as elution solvent and 0.36 g of acid alumina as cleanup reagent. The target analytes was determined by high-performance liquid chromatography. The recoveries of tanshinones obtained by analyzing the spiked samples were from 83.81% to 93.74% and relative standard deviations from 2.87% to 6.83%. Matrix solid-phase dispersion integrated the extraction and cleanup into a single step, which provides the advantages of being simple, fast and convenient. Compared with other conventional methods, the present method consumed less time and less organic solvent. The results demonstrate that this method has potential for the determination of active constituents and the quality control of traditional Chinese medicine.
Texto completo:
1
Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Fenantrenos
/
Extratos Vegetais
/
Cromatografia Líquida de Alta Pressão
/
Salvia miltiorrhiza
/
Abietanos
/
Extração em Fase Sólida
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
J Chromatogr Sci
Ano de publicação:
2017
Tipo de documento:
Article