Transgenic modification of potato pectic polysaccharides also affects type and level of cell wall xyloglucan.
J Sci Food Agric
; 97(10): 3240-3248, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-27976364
ABSTRACT
BACKGROUND:
Genes encoding pectic enzymes were introduced into wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing ß-galactosidase (ß-Gal-14) or rhamnogalacturonan lyase (RGL-18). Pectic polysaccharides from the ß-Gal-14 transgenic line exhibited rhamnogalacturonan-I structural elements with shorter galactan side chains, whereas the RGL-18 transgenic line had less rhamnogalacturonan-I structures than Karnico. Xyloglucan in primary cell walls interacts with pectin and other cell wall polysaccharides and controls cell growth.RESULTS:
Xyloglucan extracts from transgenic lines had different levels of monosaccharides compared to wild-type. Most XXGG-type xyloglucans from Karnico and RGL-18 alkali-extractable extracts predominantly consisted of XXGG and XSGG building blocks. Karnico and RGL-18 4 mol L-1 extracts had small proportions of the XXXG-type xyloglucan, whereas ß-Gal-14 extracts also contained the XXXG-type xyloglucan. The peak ratios of XSGG/XXGG were 1.9, 2.4 and 1.1 for 4 mol L-1 extracts of Karnico, RGL-18 and ß-Gal-14 lines, respectively.CONCLUSION:
After transgenic modification on pectin, the xyloglucan building blocks may have been changed. The ß-Gal-14 lines mostly present XXXG-type repeating units instead of the XXGG-type in 4 mol L-1 extracts. The ratio of XSGG/XXGG repeating units also changed, indicating that the transgenic modification of pectin altered xyloglucan structure during plant development. © 2016 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Xilanos
/
Solanum tuberosum
/
Parede Celular
/
Pectinas
/
Plantas Geneticamente Modificadas
/
Glucanos
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Holanda