Development of anti-CD47 single-chain variable fragment targeted magnetic nanoparticles for treatment of human bladder cancer.
Nanomedicine (Lond)
; 12(6): 597-613, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-28186432
ABSTRACT
AIM:
To develop a novel anti-CD47 single-chain variable fragment (scFv) functionalized magnetic nanoparticles (MNPs) for targeting bladder cell lines and its applicability in thermotherapy. MATERIAL &METHODS:
An immunized murine antibody phage display library was constructed and screened to isolate anti-CD47 binders. A scFv was selected and conjugated to MNPs which was then utilized to discriminate CD47+ bladder cells along with assessing its efficacy in thermotherapy.RESULTS:
An scFv with high affinity to bladder cells was efficiently conjugated to MNPs. Following a hyperthermia treatment, the function of scFv-MNP conjugates led to a considerable reduction in cell viability.CONCLUSION:
The anti-CD47 scFv-MNP conjugate was an effective cancer cell thermotherapy tool that might pave the way for development of bionano-based targeting techniques in both early detection and treatment of cancer.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Bexiga Urinária
/
Antígeno CD47
/
Anticorpos de Cadeia Única
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Nanopartículas de Magnetita
Tipo de estudo:
Screening_studies
Idioma:
En
Revista:
Nanomedicine (Lond)
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Irã