Development and Validation of an Enzymatic Method To Determine Stevioside Content from Stevia rebaudiana.
J Agric Food Chem
; 65(15): 3223-3229, 2017 Apr 19.
Article
em En
| MEDLINE
| ID: mdl-28343388
ABSTRACT
An enzymatic method for specific determination of stevioside content was established. Recombinant ß-glucosidase BT_3567 (rBT_3567) from Bacteroides thetaiotaomicron HB-13 exhibited selective hydrolysis of stevioside at ß-1,2-glycosidic bond to yield rubusoside and glucose. Coupling of this enzyme with glucose oxidase and peroxidase allowed for quantitation of stevioside content in Stevia samples by using a colorimetric-based approach. The series of reactions for stevioside determination can be completed within 1 h at 37 °C. Stevioside determination using the enzymatic assay strongly correlated with results obtained from HPLC quantitation (r2 = 0.9629, n = 16). The percentages of coefficient variation (CV) of within day (n = 12) and between days (n = 12) assays were lower than 5%, and accuracy ranges were 95-105%. This analysis demonstrates that the enzymatic method developed in this study is specific, easy to perform, accurate, and yields reproducible results.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Edulcorantes
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Proteínas de Bactérias
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Extratos Vegetais
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Beta-Glucosidase
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Stevia
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Ensaios Enzimáticos
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Bacteroides thetaiotaomicron
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Tailândia