Simultaneous analysis of tert-butylhydroquinone, tert-butylquinone, butylated hydroxytoluene, 2-tert-butyl-4-hydroxyanisole, 3-tert-butyl-4-hydroxyanisole, α-tocopherol, γ-tocopherol, and δ-tocopherol in edible oils by normal-phase high performance liquid chromatography.
Food Chem
; 234: 205-211, 2017 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-28551227
A normal-phase high performance liquid chromatography method for the simultaneous determination of tert-butylhydroquinone, tert-butylquinone, butylated hydroxytoluene, 2-tert-butyl-4-hydroxyanisole, 3-tert-butyl-4-hydroxyanisole, α-tocopherol, γ-tocopherol, and δ-tocopherol in edible oils was investigated. A silica column was used to separate the analytes with the gradient elution. An ultraviolet-visible detector was set at dual wavelengths mode (280 and 310nm). The column temperature was 30°C. The analytes were directly extracted with methanol. Results showed that the normal-phase high performance liquid chromatography method performed well with wide liner ranges (0.10â¼500.00µg/mL, R2>0.9998), low limits of detection and quantitation (below 0.40 and 1.21µg/mL, respectively), and good recoveries (81.38â¼102.34% in soybean oils and 83.03â¼100.79% in lard, respectively). The reduction of tert-butylquinone caused by the reverse-phase high performance liquid chromatography during the injection was avoided with the current normal-phase method. The two isomers of butylated hydroxyanisole can also be separated with good resolution.
Palavras-chave
2-tert-Butyl-4-hydroxyanisole; 2-tert-Butyl-4-hydroxyanisole (Pub Chem CID: 6932); 3-tert-Butyl-4-hydroxyanisole; 3-tert-Butyl-4-hydroxyanisole (Pub Chem CID: 8456); Butylated hydroxytoluene; Butylated hydroxytoluene (Pub Chem CID: 31404); Normal-phase high performance liquid chromatography analysis; Tocopherol; tert-Butylhydroquinone; tert-Butylhydroquinone (Pub Chem CID: 16043); tert-Butylquinone; tert-Butylquinone (Pub Chem CID: 19211); α-Tocopherol (Pub Chem CID: 14985); γ-Tocopherol (Pub Chem CID: 92729); δ-Tocopherol (Pub Chem CID: 92094)
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quinonas
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Óleos de Plantas
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Hidroxianisol Butilado
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Hidroxitolueno Butilado
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Tocoferóis
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2017
Tipo de documento:
Article