Supplementation with Lactobacillus paracasei or Pediococcus pentosaceus does not prevent diarrhoea in neonatal pigs infected with Escherichia coli F18.
Br J Nutr
; 118(2): 109-120, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28720151
ABSTRACT
Infectious diarrhoea is a worldwide problem in newborns. Optimal bacterial colonisation may enhance gut maturation and protect against pathogenic bacteria after birth. We hypothesised that lactic acid bacteria (LAB) administration prevents pathogen-induced diarrhoea in formula-fed newborns. Newborn caesarean-delivered, colostrum-deprived term piglets on parenteral nutrition for the first 15 h, were used as models for sensitive newborn infants. A commercially available probiotic strain, Lactobacillus paracasei F19 (LAP, 2·6×108 colony-forming units (CFU)/kg per d) and a novel LAB isolate, Pediococcus pentosaceus (PEP, 1·3×1010 CFU/kg per d), were administered for 5 d with or without inoculation of the porcine pathogen, Escherichia coli F18 (F18, 1010 CFU/d). This resulted in six treatment groups Controls (n 9), LAP (n 10), PEP (n 10), F18 (n 10), F18-LAP (n 10) and F18-PEP (n 10). The pathogen challenge increased diarrhoea and density of F18 in the intestinal mucosa (P<0·05). LAB supplementation further increased the diarrhoea score, relative to F18 alone (P<0·01). Intestinal structure and permeability were similar among groups, whereas brush border enzymes were affected in variable intestinal regions with decreased activities in most cases after F18 and LAB inoculation. Bacterial density in colon mucosa increased after F18 inoculation (P<0·05) but was unaffected by LAB supplementation. In colon contents, acetic and butyric acids were increased by PEP (P<0·05). The LAB used in this study failed to reduce E. coli-induced diarrhoea in sensitive newborn pigs. In vulnerable newborns there may be a delicate balance among bacterial composition and load, diet and the host. Caution may be required when administering LAB to compromised newborns suffering from enteric infections.
Palavras-chave
CFU colony-forming units; CONT control; ETEC Escherichia coli; F18 Escherichia coli F18; FISH fluorescent in situ hybridisation; LAB lactic acid bacteria; LAP Lactobacillus paracasei F19; NEC necrotising enterocolitis; PEP Pediococcus pentosaceus; SI small intestine; qPCR quantitative PCR; Enteric infections; Gut colonisation; Neonatal pigs; Probiotics
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Diarreia
/
Infecções por Escherichia coli
/
Lacticaseibacillus paracasei
/
Pediococcus pentosaceus
/
Animais Recém-Nascidos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Br J Nutr
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Dinamarca