Mechanisms underlying the wound healing potential of propolis based on its in vitro antioxidant activity.
Phytomedicine
; 34: 76-84, 2017 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-28899513
ABSTRACT
BACKGROUND:
Propolis is a resinous substance collected by honeybees, Apis mellifera, from various plant sources. Having various pharmacological and biological activities, it has been used in folk medicine and complementary therapies since ancient times.PURPOSE:
To evaluate the effects and underlying mechanism of the protective effects of the ethanol extract of Chinese propolis (EECP) on L929 cells injured by hydrogen peroxide (H2O2). STUDYDESIGN:
The wound healing activities of EECP in L929 cells with H2O2-induced damage were investigated.METHODS:
The main components of EECP were analyzed by RP-HPLC, and the free radical scavenging capacity and reducing power were also measured. The effects of EECP on the expression of antioxidant-related genes in fibroblast L929 cells were determined using qRT-PCR and western blotting.RESULTS:
EECP had significant protective effects against cell death induced by H2O2 and significantly inhibited the decline of collagen mRNA expression caused by H2O2 in L929 cells.CONCLUSION:
EECP induced the expression of antioxidant-related genes, such as HO-1, GCLM, and GCLC, which has great implications for the potential of propolis to alleviate oxidative stress in wound tissues. The protective effects of propolis have great implications for using propolis as a wound healing regent.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Própole
/
Cicatrização
/
Antioxidantes
Idioma:
En
Revista:
Phytomedicine
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China