Orthosiphon stamineus (Misai Kucing) ameliorated postmenopausal osteoporosis in rat model.
Menopause
; 25(2): 202-210, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-28926512
ABSTRACT
OBJECTIVE:
Orthosiphon stamineus (OS) or Misai Kucing (Java tea) is a popular herbal supplement from Southeast Asia for various metabolic, age-related diseases. This study investigated the potential use of OS leaf extracts to ameliorate osteoporosis in ovariectomized rats.METHODS:
Fifty-six female Sprague-Dawley rats were randomly allocated into eight groups (nâ=â7) SHAM (healthy sham control); OVX (ovarietomized) nontreated rats (negative control); OVXâ+âRemifemin (100âmg/kg body weight), and 2% green tea extract (positive controls); OVXâ+âOS 50% ethanolic and aqueous extracts, both at either 150 or 300âmg/kg. After 16 weeks, the rats' bones and blood were evaluated for osteoporosis indicators (protein and mRNA expressions), micro-computed tomography for bone histomorphometry, and three-point bending test for tibia mechanical strength.RESULTS:
The extracts dose-dependently and significantly (Pâ<â0.05) improved bone strength and flexibility, bone mineral density, bone formation protein markers (P1NP), and bone histomorphometry. All extracts reduced the inflammation biomarker (interleukin-6). The extracts up-regulated osteoblastogenesis (bone morphogenetic protein-2) and collagen-1 synthesis (collagen type 1 alpha-1) mRNA expressions, and down-regulated bone resorption (TNFSF11 and nuclear factor-kappa B) mRNA expressions. Both the water and 50% ethanolic extract were effective. The effective dose is equivalent to 25 to 50âmg/kg extract for humans.CONCLUSIONS:
The extract showed bone-protective and antiosteoporotic effects (improving bone strength, flexibility, bone density, and bone morphometry) by reducing inflammation and the bone resorption biomarkers, while enhancing bone formation biomarkers and collagen synthesis.
Texto completo:
1
Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Plantas_medicinales
Assunto principal:
Extratos Vegetais
/
Osteoporose Pós-Menopausa
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Orthosiphon
/
Chás de Ervas
Tipo de estudo:
Clinical_trials
/
Prognostic_studies
Idioma:
En
Revista:
Menopause
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Malásia