Your browser doesn't support javascript.
loading
Central noradrenergic activity affects analgesic effect of Neuropeptide S.
Jinushi, Kei; Kushikata, Tetsuya; Kudo, Takashi; Calo, Girolamo; Guerrini, Remo; Hirota, Kazuyoshi.
Afiliação
  • Jinushi K; Department of Anesthesiology, Hirosaki University Hospital, Hirosaki, 036-8563, Japan.
  • Kushikata T; Department of Anesthesiology, Hirosaki University Graduate School of Medicine, Zaifu 5, Hirosaki, 036-8562, Japan. tetsuyak@hirosaki-u.ac.jp.
  • Kudo T; Department of Anesthesiology, Hirosaki University Hospital, Hirosaki, 036-8563, Japan.
  • Calo G; Section of Pharmacology, Department of Medical Science, National Institute of Neuroscience, University of Ferrara, Ferrara, Italy.
  • Guerrini R; Department of Chemical and Pharmaceutical Sciences, LTTA, University of Ferrara, 44121, Ferrara, Italy.
  • Hirota K; Department of Anesthesiology, Hirosaki University Graduate School of Medicine, Zaifu 5, Hirosaki, 036-8562, Japan.
J Anesth ; 32(1): 48-53, 2018 02.
Article em En | MEDLINE | ID: mdl-29128909
BACKGROUND: Neuropeptide S (NPS) is an endogenous neuropeptide controlling anxiolysis, wakefulness, and analgesia. NPS containing neurons exist near to the locus coeruleus (LC) involved in the descending anti-nociceptive system. NPS interacts with central noradrenergic neurons; thus brain noradrenergic signaling may be involved in NPS-induced analgesia. We tested NPS analgesia in noradrenergic neuron-lesioned rats using a selective LC noradrenergic neurotoxin, N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4). METHODS: A total 66 male Sprague-Dawley rats weighing 350-450 g were used. Analgesic effects of NPS were evaluated using hot-plate and tail-flick test with or without DSP-4. The animal allocated into 3 groups; hot-plate with NPS alone intracerebroventricular (icv) (0.0, 1.0, 3.3, and 10.0 nmol), tail-flick NPS alone icv (0.0 and 10.0 nmol), and hot-plate with NPS and DSP-4 (0 or 50 mg/kg ip). In hot-plate with NPS and DSP-4 group, noradrenaline content in the cerebral cortex, pons, hypothalamus, were measured. RESULTS: NPS 10 nmol icv prolonged hot plate (%MPE) but not tail flick latency at 30 and 40 min after administration. DSP-4 50 mg/kg decreased noradrenaline content in the all 3 regions. The NA depletion inhibited NPS analgesic effect in the hot plate test but not tail flick test. There was a significant correlation between hot plate latency (percentage of maximum possible effect: %MPE) with NPS 10 nmol and NA content in the cerebral cortex (p = 0.017, r 2 = 0.346) which noradrenergic innervation arisen mainly from the LC. No other regions had the correlation. CONCLUSIONS: NPS analgesia interacts with LC noradrenergic neuronal activity.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dor / Benzilaminas / Norepinefrina / Analgésicos Idioma: En Revista: J Anesth Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dor / Benzilaminas / Norepinefrina / Analgésicos Idioma: En Revista: J Anesth Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão