Use of Innovative (Micro)Extraction Techniques to Characterise Harpagophytum procumbens Root and its Commercial Food Supplements.
Phytochem Anal
; 29(3): 233-241, 2018 May.
Article
em En
| MEDLINE
| ID: mdl-29143440
ABSTRACT
INTRODUCTION:
For the determination of harpagoside and the wide phenolic pattern in Harpagophytum procumbens root and its commercial food supplements, dispersive liquid-liquid microextraction (DLLME), ultrasound-assisted DLLME (UA-DLLME), and sugaring-out liquid-liquid extraction (SULLE) were tested and compared.OBJECTIVES:
In order to optimise the extraction efficiency, DLLME and UA-DLLME were performed in different solvents (water and aqueous solutions of glucose, ß-cyclodextrin, (2-hydroxypropyl)-ß-cyclodextrin, sodium chloride, natural deep eutectic solvent, and ionic liquid). MATERIAL ANDMETHODS:
The plant material was ground and sieved to obtain a uniform granulometry before extraction. Commercial food supplements, containing H. procumbens are commercially available in Italy.RESULTS:
The most effective sodium chloride-aided-DLLME was then optimised and applied for analyses followed by HPLC-PDA. For comparison, microwave-assisted extraction was performed using the same solvents and the best results were obtained using 1% of ß-cyclodextrin or 15% of sodium chloride.CONCLUSION:
All commercial samples respected the European Pharmacopoeia monograph for this plant material, showing a harpagoside content ≥ 1.2%. Copyright © 2017 John Wiley & Sons, Ltd.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Piranos
/
Raízes de Plantas
/
Suplementos Nutricionais
/
Harpagophytum
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Microextração em Fase Líquida
/
Glicosídeos
País/Região como assunto:
Europa
Idioma:
En
Revista:
Phytochem Anal
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Eslováquia