In vivo and in vitro neuroprotective effects of Panax ginseng glycoproteins.
Int J Biol Macromol
; 113: 607-615, 2018 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-29408615
ABSTRACT
The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function. A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed. The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa. HPLC analysis revealed that the compositions of glycoproteins included fucose, mannose, rhamnose, glucose, galacturonic acid, N-acetylglucosamine and N-acetylgalactosamine. Glycan of PGL-1 has a backbone of â4)-Rha-(1â, â4)-Fuc -(1â, â6)-Gal-(1â, â4)-GalA-(1â, â4)-GlcNAc-(1â and â4)-GalNAc-(1â,and (â3,6)-Man-(1â) was distributed in branches. The (1â)-Fuc, (1â)-Glc and (1â)-GlcNAc or (1â)-GalNAc were regarded as a terminal residue. The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aß25-35. All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aß25-35, and obviously inhibited the S phase arrest. Compared with Aß25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins. Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.
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Base de dados:
MEDLINE
Medicinas Complementares:
Homeopatia
Assunto principal:
Glicoproteínas
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Fármacos Neuroprotetores
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Panax
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China