Screening of bioactive components from traditional Chinese medicines using cell membrane chromatography coupled with mass spectrometry.
Phytochem Anal
; 29(4): 341-350, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29573482
ABSTRACT
INTRODUCTION:
Cell membrane chromatography (CMC), as a highly selective type of affinity chromatography, has been demonstrated as an effective method to screen bioactive components acting on specific receptor from a complicated biological system.OBJECTIVE:
To review the recent research progress and the technical applications of these analytical methods using CMC combined with gas chromatography-mass spectrometry, (GC/MS) and liquid chromatography-mass spectrometry (LC/MS).METHODOLOGY:
In this review, we briefly introduce the CMC offline GC/MS, CMC online GC/MS, CMC offline LC/MS, and CMC online LC/MS system. And the practical application of these technologies is also enumerated. Then the future of these technologies and research methods were discussed.RESULTS:
Many bioactive components interacting with specific receptors have been screened and identified in traditional Chinese medicines.CONCLUSION:
CMC technique has been combined with GC/MS and HPLC/MS and these combined systems have been successfully used to screen bioactive components acting on specific receptors from a complicated biological system.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Cromatografia Líquida
/
Cromatografia Gasosa-Espectrometria de Massas
/
Medicina Tradicional Chinesa
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Phytochem Anal
Ano de publicação:
2018
Tipo de documento:
Article