A time course study of glucose levels and innate immune response in gilthead seabream (Sparus aurata L.) after exposure to clove oil-eugenol derived anaesthetic.
Fish Shellfish Immunol
; 77: 280-285, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29609029
ABSTRACT
Clove oil is used as an anaesthetic for many species of fish worldwide; however, relatively few studies have assessed its effects on the innate immune response on these species. The present work aimed to investigate the effects of clove oil-eugenol derived anaesthetic on some humoral and cellular immune response in gilthead seabream (Sparus aurata L.). To compare with an unexposed control group, fish were exposed to 55â¯ppm clove oil for 5â¯min, before being sampled at 1, 24 and 48â¯h post-exposure. Serum glucose level was also measured to obtain information on the fish physiological response after clove oil anaesthesia. One hour after exposure the haemolytic complement activity of fish was lower than in the unexposed group. By contrast, the leucocyte peroxidase activity in head-kidney was significantly stimulated 24â¯h after exposure to clove oil-eugenol. The rest of innate immune parameters evaluated and the glucose levels not were affected by clove oil exposure at any sampling point. Overall, the use of clove oil at 55â¯ppm as anaesthetic did not seem to alter the innate immune response and neither did it trigger a stress response. The use of clove oil-eugenol derived had become common practice in aquaculture, and its use with gilthead seabream can be considered safe as it does not cause immunodepression in anesthetized fish.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicemia
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Eugenol
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Dourada
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Óleo de Cravo
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Imunidade Inata
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Anestésicos
Idioma:
En
Revista:
Fish Shellfish Immunol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Tunísia