Juglanin ameliorates UVBinduced skin carcinogenesis via antiinflammatory and proapoptotic effects in vivo and in vitro.
Int J Mol Med
; 42(1): 41-52, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29620254
ABSTRACT
Ultraviolet (UV) radiation induces skin injury, and is associated with the development and formation of melanoma, which is a highly lethal form of skin cancer. Juglanin is a natural product, which is predominantly extracted from Polygonum aviculare, and is considered a functional component among its various compounds. Juglanin has been reported to exert marked protective effects in various diseases via the inhibition of inï¬ammation and tumor cell growth. The present study aimed to explore the effects of juglanin on human skin cancer induced by UV and to reveal the underlying molecular mechanism. In the present study, immunohistochemical analysis, western blot analysis, RT-qPCR analysis and flow cytometry assays were mainly used in vivo and/or in vitro. The results indicated that in mice, UVB exposure increased susceptibility to carcinogens, and accelerated disease pathogenesis. Conversely, juglanin was able to ameliorate this condition via inhibition of inflammation, suppression of cell proliferation and induction of apoptosis via p38/cJun Nterminal kinase (JNK) blockage, nuclear factor (NF)κB inactivation and caspase stimulation in vivo. In addition, in vitro, the present study demonstrated that treatment of UVBstimulated B16F10 melanoma cells with juglanin resulted in a dosedependent decrease in cell viability, as well as increased apoptosis via the upregulation of caspase expression and poly (ADPribose) polymerase cleavage. In addition, juglanin markedly attenuated p38/JNK signaling, inactivated the phosphoinositide 3kinase/protein kinase B pathway and suppressed UVBinduced NFκB activation. Taken together, these results indicated the possibility of applying juglanin in combination with UVB as a potential therapeutic strategy for preventing skin cancer.
Texto completo:
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Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Plantas_medicinales
Assunto principal:
Neoplasias Cutâneas
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Raios Ultravioleta
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Apoptose
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Quempferóis
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Carcinogênese
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Glicosídeos
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Anti-Inflamatórios
Idioma:
En
Revista:
Int J Mol Med
Ano de publicação:
2018
Tipo de documento:
Article