[Protective effect of catalpolon destruction of tight junctions of high glucose induced BMECs].
Zhongguo Zhong Yao Za Zhi
; 43(20): 4118-4124, 2018 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-30486540
ABSTRACT
This paper aimed to observe the protective effect of catalpol on the high glucose induced destruction of tight junctions of rat primary brain microvascular endothelial cells (BMECs). Catalpol co-administrated with high glucose increased BMECs survival, decreased its ET-1 secretion, and improved transmembrane electrical resistance in a time-dependent manner. Furthermore, transmission electron microscopy was used to observe catalpol's protective effect on tight junction. Fluorescence staining displayed that catalpol reversed the rearrangement of the cytoskeleton protein F-actin and up-regulated the tight junction proteins claudin-5 and ZO-1, which were further demonstrated by the mRNA expression levels of claudin-5, occludin, ZO-1, ZO-2, ZO-3, α-actintin, vinculin and cateinins. This study indicated that catalpol reverses the disaggregation of cytoskeleton actin in BMECs and up-regulates the expression of tight junction proteins, such as claudin-5, occludin, and ZO-1, and finally alleviates the increase in high glucose-induced BMECs injury.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto de Actina
/
Junções Íntimas
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Células Endoteliais
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Glucosídeos Iridoides
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China