O<sub>2</sub>-tolerant [NiFe]-hydrogenases of Ralstonia eutropha H16: Physiology, molecular biology, purification, and biochemical analysis.

Lenz, Oliver; Lauterbach, Lars; Frielingsdorf, Stefan

O₂-tolerant [NiFe]-hydrogenases of Ralstonia eutropha H16: Physiology, molecular biology, purification, and biochemical analysis.

Lenz, Oliver; Lauterbach, Lars; Frielingsdorf, Stefan.

Afiliação

Lenz O; Department of Chemistry, Technische Universität Berlin, Berlin, Germany. Electronic address: oliver.lenz@tu-berlin.de.
Lauterbach L; Department of Chemistry, Technische Universität Berlin, Berlin, Germany.
Frielingsdorf S; Department of Chemistry, Technische Universität Berlin, Berlin, Germany.

Methods Enzymol ; 613: 117-151, 2018.

Article em En | MEDLINE | ID: mdl-30509463

Assuntos

Cupriavidus necator/enzimologia; Cupriavidus necator/metabolismo; Hidrogenase/metabolismo; Oxigênio/metabolismo; Catálise; Cromatografia Gasosa; Hidrogênio/metabolismo; Oxirredução; Plasmídeos/genética

Palavras-chave

Affinity chromatography; Alcaligenes eutrophus; Biofuel cell; Catalytic hydrogendeuterium exchange; Chemolithotrophy; Clark electrode; Cofactor recycling; Cupriavidus necator; Enzyme purification; Genetic manipulation; Hydrogen oxidation; Hydrogen production; Hydrogenase; Hydrogenomonas; Iron; Ironsulfur clusters; Knallgas; Metalloenzyme; Nickel; Oxygen tolerance; Photometric assay; Plasmid; Protein engineering; Ralstonia eutropha; Wautersia eutropha; [NiFe]-hydrogenase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigênio / Cupriavidus necator / Hidrogenase Idioma: En Revista: Methods Enzymol Ano de publicação: 2018 Tipo de documento: Article

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