Evaluation of an extract of the Punica granatum flower as a biological stain of rat tissues: a preliminary study.

ABSTRACT

Natural dyes and especially hematoxylin, which is herbal, are widely used in staining tissues. The aim of this study is to evaluate the staining status of different tissues taken from rats with pomegranate flower extract. For this reason, 2 Wistar albino rats, one male and one female, were used as stain biomaterial. A histological follow up procedure was applied to the lung, kidney, liver, and heart tissue samples taken from the rats and the unstained preparates of these tissues were prepared. As the source of the dye, the dry flowers of Punica granatum (PG) obtained from local markets of Kayseri were used. Each tissue sample underwent the same staining procedure with the same temperature, duration, and dye solution. Before and after the staining procedure, × 40 images of the tissue preparates were taken using a light microscope. Generally, different tones of staining were observed in the nuclei and cytoplasms of all cells and epithelium cells. Staining in parts specific to each tissue occurred. For example, there were light stains on the glomerular cells and the Bowman capsule in the kidney tissue Differences in staining can only be explained by molecular diversity differences in tissue. However, in order to improve the initial staining results obtained in this study, it is possible that working with different temperatures, pH values, mordant substances, and dye that the dye molecules in the extract will provide more vivid colors with different molecules in the tissues.