Evaluation of the Chemopreventive Activity of Grape Skin Extract Using Medium-term Oral Carcinogenesis Assay Induced by 4-Nitroquinoline 1-Oxide.
Anticancer Res
; 39(1): 177-182, 2019 Jan.
Article
em En
| MEDLINE
| ID: mdl-30591456
ABSTRACT
BACKGROUND/AIM:
The aim of this study was to evaluate the chemoprotective potential of grape skin extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). MATERIALS ANDMETHODS:
Male Wistar rats were distributed into four groups (n=5, per group) Control Group free access to commercial diet and drinking water for 12 weeks; 4NQO Group received 4NQO diluted in drinking water daily, for 12 weeks; Grape Skin Extract Group free access to water and received grape skin extract incorporated with diet for 12 weeks; 4NQO + Grape Skin Extract Group received 4NQO in drinking water daily and grape extract incorporated with diet for 12 weeks.RESULTS:
Animals treated with grape skin extract revealed a significant reduction in epithelial dysplasia. Also, 8-hydroxy-2'-deoxyguanosine (8-OHdG) and ki-67 immunoexpression was reduced in animals treated with grape skin extract. Western blot analysis showed a significant decrease of p-NFκB p50 and MyD88 protein expression in the groups treated with grape skin extract. Copper-zinc superoxide dismutase, manganese superoxide dismutase, and catalase gene expression did not present any statistically significant differences (p>0.05).CONCLUSION:
Grape skin extract displayed chemopreventive activity in oral carcinogenesis assays as depicted by its antioxidant, anti-proliferative and anti-inflammatory properties.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Bucais
/
Extratos Vegetais
/
Vitis
/
Carcinogênese
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil