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Induction of oxidative stress, apoptosis and DNA damage by koumine in Tetrahymena thermophila.
Ye, Qiao; Zhang, Chaonan; Wang, Zhenlu; Feng, Yongyong; Zhou, Aiguo; Xie, Shaolin; Xiang, Qiong; Song, Enfeng; Zou, Jixing.
Afiliação
  • Ye Q; Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Zhang C; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Wang Z; Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Feng Y; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Zhou A; Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Xie S; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Xiang Q; Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Song E; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
  • Zou J; Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
PLoS One ; 14(2): e0212231, 2019.
Article em En | MEDLINE | ID: mdl-30753239
ABSTRACT
Koumine is a component of the Chinese medicinal herb Gelsemium elegans and is toxic to vertebrates. We used the ciliate Tetrahymena thermophila as a model to evaluate the toxic effects of this indole alkaloid in eukaryotic microorganisms. Koumine inhibited T. thermophila growth and viability in a dose-dependent manner. Moreover, this drug produced oxidative stress in T. thermophila cells and expressions of antioxidant enzymes were significantly elevated at high koumine levels (p < 0.05). Koumine also caused significant levels of apoptosis (p < 0.05) and induced DNA damage in a dose-dependent manner. Mitophagic vacuoles were present in cells indicating induction of autophagy by this drug. Expression of ATG7, MTT2/4, CYP1 and HSP70 as well as the MAP kinase pathway gene MPK1 and MPK3 were significantly altered after exposed to koumine. This study represents a preliminary toxicological evaluation of koumine in the single celled eukaryote T. thermophila.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dano ao DNA / Tetrahymena thermophila / Apoptose / Estresse Oxidativo / Alcaloides Indólicos Idioma: En Revista: PLoS One Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dano ao DNA / Tetrahymena thermophila / Apoptose / Estresse Oxidativo / Alcaloides Indólicos Idioma: En Revista: PLoS One Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China