Long-term functional maintenance of primary human hepatocytes in vitro.
Science
; 364(6438): 399-402, 2019 04 26.
Article
em En
| MEDLINE
| ID: mdl-31023926
ABSTRACT
The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cultura de Vírus
/
Vírus da Hepatite B
/
Hepatócitos
/
Cultura Primária de Células
Idioma:
En
Revista:
Science
Ano de publicação:
2019
Tipo de documento:
Article