The fruit of Crataegus pinnatifida ameliorates memory deficits in ß-amyloid protein-induced Alzheimer's disease mouse model.
J Ethnopharmacol
; 243: 112107, 2019 Oct 28.
Article
em En
| MEDLINE
| ID: mdl-31349027
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE The fruit of Crataegus pinnatifida is a traditional medicine widely used as digestive drug in East Asia. Although Chinese herbal medicine used it for mental health, scientific evidence does not exist, yet. AIMS OF STUDY The aim of this study is to show that the ethanol extract of the fruit of Crataegus pinnatifida (CPE) has neuroprotective effect on Alzheimer' disease model mice. MATERIALS AND METHODS:
Intracerebroventricular injection of Aß was used to induce Alzheimer's disease-like pathology. Passive avoidance and Y-maze tasks were used to examine the effect of CPE on memory impairments by Aß. Immunohistochemistry was used to examine the effect of CPE on glial activation. ThT assay was used to observe the effect of CPE on Aß aggregation. MTT and LDH release assays were utilized to examine effects of CPE on Aß-induced cytotoxicity.RESULTS:
CPE prevented memory deficit in Aß-induced memory impairment model. Moreover, CPE prevented glial activation in the hippocampus of Aß-injected model. In in vitro test, CPE inhibited Aß fibril formation in a concentration-dependent manner. CPE also caused disaggregation of Aß fibrils. Along with this, CPE blocked neuronal cell death induced by Aß.CONCLUSIONS:
Collectively, these experimental findings demonstrated that CPE could be a candidate for development of AD therapy.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Plantas_medicinales
Assunto principal:
Extratos Vegetais
/
Fármacos Neuroprotetores
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Crataegus
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Doença de Alzheimer
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2019
Tipo de documento:
Article