[Quality evaluation of multi-components simultaneous determination and fingerprint of Gardeniae Fructus from different regions].
Zhongguo Zhong Yao Za Zhi
; 44(18): 4026-4033, 2019 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-31872741
ABSTRACT
An analysis method was established by UPLC fingerprint and then applied to simultaneous determination of multiple compounds in Gardeniae Fructus from different areas in China. Samples were separated on a Waters Acquity UPLC BEH C18( 2. 1 mm×50 mm,1. 7 µm) column with 0. 1% formic acid-water and acetonitrile solution as gradient mobile phase at a flow rate of 0. 4 m L·min-1 at various wavelengths. The similarity of samples was over 0. 95 with â³Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine( 2012 edition) â³. The UPLC common fingerprints for 32 batches were established with 19 common peaks identified. The samples were divided into 3 groups analyzed by HCA and PCA. Five components were identified as the main compositions which caused the differences of chemical constituents in the samples from different areas with partial least squares discriminant analysis( PLS-DA). The content of the total components in each area was Zhejiang > Fujian > Jiangxi > Sichuan. This method was accurate and viable,could be used to evaluate the quality of Gardeniae Fructus.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Medicamentos de Ervas Chinesas
/
Gardenia
/
Compostos Fitoquímicos
/
Frutas
Tipo de estudo:
Prognostic_studies
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China