Evidence Supporting That RNA Polymerase II Catalyzes De Novo Transcription Using Potato Spindle Tuber Viroid Circular RNA Templates.
Viruses
; 12(4)2020 03 27.
Article
em En
| MEDLINE
| ID: mdl-32230827
ABSTRACT
Transcription is a fundamental process that mediates the interplay between genetic information and phenotype. Emerging evidence indicates that RNA polymerase II (Pol II) can catalyze transcription using both DNA and RNA templates. It is well established that Pol II initiates de novo transcription on DNA templates. However, it is unclear whether Pol II performs de novo transcription or relies on primers for initiation (primed transcription) on RNA templates. Using potato spindle tuber viroid (PSTVd) as a model, we presented evidence showing that circular PSTVd templates are critical for the synthesis of longer-than-unit-length (-)-strand products, which supports the de novo transcription based on the asymmetric rolling circle model of PSTVd replication. We further showed that the crucial factor for primed transcription, transcription factor IIS (TFIIS), is dispensable for PSTVd replication in cells. Together, our data support the de novo transcription on PSTVd RNA templates catalyzed by Pol II. This result has significant implications in understanding the mechanism and machinery underlying Pol II-catalyzed transcription using other RNA templates.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
/
Moldes Genéticos
/
Transcrição Gênica
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Viroides
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RNA Polimerase II
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RNA Viral
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RNA Circular
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Viruses
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos