Genetic and molecular characterization of multicomponent resistance of Pseudomonas against allicin.
Life Sci Alliance
; 3(5)2020 05.
Article
em En
| MEDLINE
| ID: mdl-32234751
ABSTRACT
The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant Pseudomonas fluorescens (PfAR-1), which was isolated from garlic, conferred allicin resistance to Pseudomonas syringae and even to Escherichia coli Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in PfAR-1, achieved through horizontal gene transfer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas
/
Ácidos Sulfínicos
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Farmacorresistência Bacteriana
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Dissulfetos
Idioma:
En
Revista:
Life Sci Alliance
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Alemanha