Notch signaling pathway mediates the immunomodulatory mechanism of Yangfei Huoxue decoction alleviating bleomycin-induced pulmonary fibrosis in rats.
J Tradit Chin Med
; 40(2): 204-211, 2020 04.
Article
em En
| MEDLINE
| ID: mdl-32242386
ABSTRACT
OBJECTIVE:
To investigate the immunomodulatory mechanism by which Yangfei Huoxue decoction (YHD) alleviates bleomycin (BLM)-induced pulmonary fibrosis (PF) in rats.METHODS:
Rats were randomly divided into two time-point groups (day 14 and 28), and each time-point group comprised the following six subgroups control, BLM, dexamethasone (DXM), YHD high dose (YHD-H), YHD middle dose (YHD-M), and YHD low dose (YHD-L). Haematoxylin and eosin and Masson staining, flow cytometry, enzyme-linked immunosorbent assay, Western blotting and UPLC-QT of analyses were examined.RESULTS:
The results showed that YHD reduced the degree of alveolar inflammation and fibrosis; downregulated the expression of CD28, CD80, CD86, Delta-like 1, Notch2, and Notch3; and upregulated the proportions of Th1/Th2 and Tc1/Tc2. The seven components of YHD were detected.CONCLUSION:
The current study indicates that YHD mainly functions by regulating the immune system and that the molecular mechanism may be related to the regulation of the Notch signaling pathway.Palavras-chave
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Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Fibrose Pulmonar
/
Bleomicina
/
Medicamentos de Ervas Chinesas
/
Transdução de Sinais
/
Receptor Notch2
/
Receptor Notch3
Idioma:
En
Revista:
J Tradit Chin Med
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China