Bioassay-guided isolation and evaluation of anti-osteoporotic polysaccharides from Morinda officinalis.
J Ethnopharmacol
; 261: 113113, 2020 Oct 28.
Article
em En
| MEDLINE
| ID: mdl-32668320
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Morinda officinalis is a well-known Chinese tonic herb that has shown clinical efficacy in the treatment of bone disease. However, its anti-osteoporotic potential and the M. officinalis polysaccharides (MOPs) responsible for activity require further investigation. AIM OF THE STUDY This study aimed to investigate the anti-osteoporotic effects of different MOP fractions in ovariectomized (OVX) rats, and to identify the osteoprotective components by bioassay-guided isolation. MATERIALS AND METHODS:
MOPs were prepared by hot water and alkali extraction, separated into three fractions (MO50, MO70, and MOB) and evaluated in the classic OVX rat model and in MC3T3-E1 cells for anti-osteoporotic activity.RESULTS:
Administration of MOPs (400â¯mg/kg/day) provided significant protection against ovariectomy-induced bone loss and biomechanical dysfunction in rats. Treated animals exhibited reduced deterioration of trabecular microarchitecture and lower levels of bone turnover markers. Bioactivity-guided fractionation led to the isolation of two inulin-type fructans from MO50, MOW50-1 and MOP50-2, with potential anti-osteoporotic activities. These consisted of (2â¯ââ¯1)-linked ß-D-fructosyl residues with degrees of polymerization (DP) of 7 and 13, respectively. Furthermore, MOW50-1 promoted osteogenic differentiation of MC3T3-E1 cells by increasing alkaline phosphatase activity.CONCLUSIONS:
These data suggest very strongly that MOPs, especially MO50 and MOW50-1, may play important roles in the prevention and treatment of osteoporosis.Palavras-chave
MOP50-2; MOW50-1; MOW50-1 and MOP50-2 are inulin-type fructans with a backbone consisting of α-D-Glcp-(1â, â1)-ß-D-Fruf-(2â and ß-D-Fruf-(2â residues (DPâ¯=â¯7 and 13, respectively); Morinda officinalis How; Osteoporosis; Ovariectomized rats; Polysaccharide; Structural characterization
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
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Polissacarídeos
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Bioensaio
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Osteoporose Pós-Menopausa
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Remodelação Óssea
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Morinda
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Conservadores da Densidade Óssea
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Fêmur
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Vértebras Lombares
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China