Utilizing Potato Virus X to Monitor RNA Movement.
Methods Mol Biol
; 2166: 181-194, 2020.
Article
em En
| MEDLINE
| ID: mdl-32710409
ABSTRACT
Mobility assays coupled with RNA profiling have revealed the presence of hundreds of full-length non-cell-autonomous messenger RNAs that move through the whole plant via the phloem cell system. Monitoring the movement of these RNA signals can be difficult and time consuming. Here we describe a simple, virus-based system for surveying RNA movement by replacing specific sequences within the viral RNA genome of potato virus X (PVX) that are critical for movement with other sequences that facilitate movement. PVX is a RNA virus dependent on three small proteins that facilitate cell-to-cell transport and a coat protein (CP) required for long-distance spread of PVX. Deletion of the CP blocks movement, whereas replacing the CP with phloem-mobile RNA sequences reinstates mobility. Two experimental models validating this assay system are discussed. One involves the movement of the flowering locus T RNA that regulates floral induction and the second involves movement of StBEL5, a long-distance RNA signal that regulates tuber formation in potato.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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RNA Viral
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Clonagem Molecular
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Potexvirus
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RNA de Plantas
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Floema
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Reação em Cadeia da Polimerase em Tempo Real
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China