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Identification and characterization of fungi associated with blister blight lesions of tea (Camellia sinensis L. Kuntze) isolated from Meghalaya, India.
Barman, Ananya; Nath, Archana; Thakur, Debajit.
Afiliação
  • Barman A; Microbial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, 781035 Assam, India.
  • Nath A; Microbial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, 781035 Assam, India.
  • Thakur D; Microbial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, 781035 Assam, India. Electronic address: debajitthakur@iasst.gov.in.
Microbiol Res ; 240: 126561, 2020 Nov.
Article em En | MEDLINE | ID: mdl-32799070
Diseases in plants are mostly caused by fungi. Fungal interactions with the host can be either biotrophic, necrotrophic or hemibiotrophic. Synergistic polymicrobial interactions have been recently recognized that can also attribute to the occurrence of complex plant diseases. Tea is one of the most widely consumed beverages worldwide, although tea plants are affected by many different diseases causing a significant reduction in global tea production. Blister blight is one such serious and damaging leaf disease of tea. An assessment of blister blight disease was carried out at the tea development center in Umsning, Meghalaya. A considerable number of tea varieties showed characteristic blister blight symptoms that ranged from preliminary yellow spots in the upper leaf surface, matured white sporulating blisters in the lower leaf surface, and delayed brown necrotic lesions throughout the surfaces of the leaves. A total of 42 isolates, 15 from initial, 15 from mature, and 12 from necrotic stages were isolated from the symptomatic leaf samples. Pestalotiopsis and Nigrospora were the two fungi incessantly isolated from the diseased leaves. Colony characteristics that included colony, hyphal, and spore morphologies were examined and mycelial accumulation, sporulation, and sporal germination were determined for all the isolates of Pestalotiopsis and Nigrospora. Molecular analysis based on ITS-RFLP was performed for identification and genetic variability. In vitro pathogenicity assay revealed that Pestalotiopsis spp. and Nigrospora sp. developed distinct characteristics symptoms on greenhouse acclimated TV17 tea clones. To our knowledge, this is the first report of the prevalence of tea blister blight disease in Meghalaya and it is an initial attempt to identify fungal pathogens during different stages of blister blight disease.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Ascomicetos / Folhas de Planta / Camellia sinensis Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies País/Região como assunto: Asia Idioma: En Revista: Microbiol Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Ascomicetos / Folhas de Planta / Camellia sinensis Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies País/Região como assunto: Asia Idioma: En Revista: Microbiol Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Índia