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Preliminary study of Yinhuapinggan granule against H1N1 influenza virus infection in mice through inhibition of apoptosis.
Du, Hai-Xia; Zhou, Hui-Fen; Yang, Jie-Hong; Lu, Yi-Yu; He, Yu; Wan, Hai-Tong.
Afiliação
  • Du HX; College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China.
  • Zhou HF; College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
  • Yang JH; College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China.
  • Lu YY; Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou, China.
  • He Y; College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
  • Wan HT; College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China.
Pharm Biol ; 58(1): 979-991, 2020 Dec.
Article em En | MEDLINE | ID: mdl-32962483
CONTEXT: Yinhuapinggan granule (YHPG) is frequently used for treating fever, cough, and viral pneumonia in traditional Chinese medicine. OBJECTIVE: This study investigated the antiviral effects of YHPG in H1N1 influenza virus (IFV)-infected mice and its possible mechanism. MATERIALS AND METHODS: ICR mice were intranasally infected with 10 LD50 viral dose of IFV and then oral administration of YHPG (6, 12, and 18 g/kg) or oseltamivir (positive control) once a day for 2 or 4 consecutive days, six mice in each group. The lung, spleen and thymus indexes of IFV-infected mice, the expression of viral loads and pathological changes in lung tissues were performed to evaluate the antiviral effects of YHPG. Real-time PCR, immunohistochemistry and western blot assays were used to determine the expression of Bax, Bcl-2 and caspase-3. RESULTS: LD50 in mice was 10-3.5/0.02 mL. YHPG (6, 12, and 18 g/kg) dose-dependently decreased the lung index and viral load; the inhibition ratio of lung index was 5.31, 18.22, and 34.06%, respectively. Further detection revealed that YHPG (12 and 18 g/kg) significantly attenuated lung pathological changes, and increased the spleen and thymus indexes. Moreover, YHPG significantly down-regulated the mRNA and protein expression of Bax and caspase-3 in lung tissues of mice infected with IFV, and up-regulated the expression of Bcl-2. CONCLUSIONS: YHPG has significant antiviral effects in IFV-infected mice, partially by inhibiting influenza virus replication and regulating the occurrence of apoptosis induced by influenza virus infection, suggesting that YHPG may be a promising antiviral agent with potential clinical application prospects.
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Texto completo: 1 Base de dados: MEDLINE Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapêuticos e Terapias MTCI: Terapias_biologicas Assunto principal: Antivirais / Medicamentos de Ervas Chinesas / Infecções por Orthomyxoviridae / Vírus da Influenza A Subtipo H1N1 Tipo de estudo: Diagnostic_studies Idioma: En Revista: Pharm Biol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapêuticos e Terapias MTCI: Terapias_biologicas Assunto principal: Antivirais / Medicamentos de Ervas Chinesas / Infecções por Orthomyxoviridae / Vírus da Influenza A Subtipo H1N1 Tipo de estudo: Diagnostic_studies Idioma: En Revista: Pharm Biol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China