Dietary omega-3 fatty acids prevent neonatal seizure-induced early alterations in the hippocampal glutamatergic system and memory deficits in adulthood.
Nutr Neurosci
; 25(5): 1066-1077, 2022 May.
Article
em En
| MEDLINE
| ID: mdl-33107813
ABSTRACT
OBJECTIVE:
We investigated the influence of dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) on glutamatergic system modulation after a single episode of neonatal seizures and their possible effects on seizure-induced long-lasting behavioral deficits.METHODS:
Male Wistar rats receiving an omega-3 diet (n-3) or an n-3 deficient diet (D) from the prenatal period were subjected to a kainate-induced seizure model at P7. Glutamate transporter activity and immunocontents (GLT-1 and GLAST) were assessed in the hippocampus at 12, 24, and 48 h after the seizure episode. Fluorescence intensity for glial cells (GFAP) and neurons (NeuN) was assessed 24â h after seizure in the hippocampus. Behavioral analysis (elevated-plus maze and inhibitory avoidance memory task) was performed at 60 days of age.RESULTS:
The D group showed a decrease in glutamate uptake 24â h after seizure. In this group only, the GLT1 content increased at 12â h, followed by a decrease at 24â h. GLAST increased up to 24â h after seizure. GFAP fluorescence was higher, and NeuN fluorescence decreased, in the D group independent of seizures. In adulthood, the D group presented memory deficits independent of seizures, but short-term memory (1.5â h after a training session) was abolished in the D group treated with kainate.SIGNIFICANCE:
N-3 PUFA positively influenced the glutamatergic system during seizure and prevented seizure-related memory deficits in adulthood.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Ômega-3
/
Epilepsia
Idioma:
En
Revista:
Nutr Neurosci
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Brasil