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Core epitope analysis of 16 kDa allergen from tartary buckwheat.
Zheng, Bei; Zhang, Haina; Shen, Wei; Wang, Lei; Chen, Peng.
Afiliação
  • Zheng B; Laboratory of Biochemistry and Molecular Biology, College of Life Science, Northwest A&F University, Yangling, China.
  • Zhang H; Laboratory of Biochemistry and Molecular Biology, College of Life Science, Northwest A&F University, Yangling, China.
  • Shen W; Laboratory of Biochemistry and Molecular Biology, College of Life Science, Northwest A&F University, Yangling, China.
  • Wang L; Laboratory of Biochemistry and Molecular Biology, College of Life Science, Northwest A&F University, Yangling, China.
  • Chen P; Laboratory of Biochemistry and Molecular Biology, College of Life Science, Northwest A&F University, Yangling, China. Electronic address: pengchen@nwsuaf.edu.cn.
Food Chem ; 346: 128953, 2021 Jun 01.
Article em En | MEDLINE | ID: mdl-33412487
Tartary buckwheat is widely accepted as its nutritionalvalue. Some allergic reactions hinder its utilization. This research focused on evaluating the core epitope of 16 kDa allergen (Fag t 2) in tartary buckwheat. Six B- and seven T cell epitopes of Fag t 2 were predicted, and six B cell epitope-mutants were expressed in Pichia pastoris. Bioinformatics analysis and SDS-PAGE demonstrated that the molecular weight, isoelectric point and spatial structures of six mutant allergens were similar with Fag t 2, with the same signal peptide sequences and α-amylase inhibitor domain. There was no significant change in mutants' spatial conformation confirmed by Circular Dichroism. The position K132N and peptides at 108-117 and 132-141 were the core B- and T cell epitopes of Fag t 2 confirmed by competitive inhibition ELISA and dot blot. This result was of great significance on the study of allergen epitopes in prevention and treatment of hypersensitivity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alérgenos / Fagopyrum / Epitopos Idioma: En Revista: Food Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alérgenos / Fagopyrum / Epitopos Idioma: En Revista: Food Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China