Rutin prevents inflammation induced by lipopolysaccharide in RAW 264.7 cells via conquering the TLR4-MyD88-TRAF6-NF-κB signalling pathway.
J Pharm Pharmacol
; 73(1): 110-117, 2021 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-33791807
ABSTRACT
OBJECTIVES:
Inflammation widely exists in many diseases and poses a great threat to human and animal health. Rutin, quercetin-3-rhamnosyl glucoside, has a variety of pharmacological effects, including anti-oxidant, anti-inflammatory, antibacterial, anticancer and radioresistance effects. The current study focused on evaluation of its anti-inflammatory activity and described the mechanism of rutin in lipopolysaccharide-induced RAW 264.7 cells.METHODS:
The related gene and protein expression levels were investigated by quantification real-time PCR and western blotting, respectively. KEYFINDINGS:
This study revealed that rutin can decrease inducible nitric oxide synthase (iNOS) gene and protein expression levels, effectively increase IκB gene expression, reduce toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor-associated factor 6 (TRAF6) and p65 gene expression and inhibit the phosphorylation of IκB and p65 and the proteins expression of TLR4, MyD88 and TRAF6.CONCLUSIONS:
These results suggest that rutin might exert anti-inflammatory effect on LPS-stimulated RAW 264.7 cells and will be potentially useful as an adjuvant treatment for inflammatory diseases.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Rutina
/
NF-kappa B
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Fator 6 Associado a Receptor de TNF
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Receptor 4 Toll-Like
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Fator 88 de Diferenciação Mieloide
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Inflamação
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Anti-Inflamatórios
Idioma:
En
Revista:
J Pharm Pharmacol
Ano de publicação:
2021
Tipo de documento:
Article