Protocol for the Production of Doubled Haploid Plants of Brassica carinata.
Methods Mol Biol
; 2288: 163-180, 2021.
Article
em En
| MEDLINE
| ID: mdl-34270011
ABSTRACT
Brassica carinata, also known as Ethiopian or Abyssinian mustard, is a drought- and heat-tolerant oilseed with great potential as a dedicated industrial feedstock crop for use in biofuel and other bio-based applications. Doubled haploid technology, a system that allows for the rapid development of doubled haploid, completely homozygous plants through microspore embryogenesis, has been applied routinely in both B. carinata breeding and basic research. Here, we present a comprehensive isolated microspore culture protocol detailing the various steps involved in doubled haploid plant production for this species, from growing donor plants over harvesting flower buds and isolating, culturing and inducing microspores to regenerating doubled haploid embryos and plantlets.
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1
Base de dados:
MEDLINE
Assunto principal:
Melhoramento Vegetal
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Mostardeira
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Canadá