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Morin attenuates osteoclast formation and function by suppressing the NF-κB, MAPK and calcium signalling pathways.
Shi, Yifeng; Ye, Lin; Shen, Shiwei; Qian, Tianchen; Pan, Youjin; Jiang, Yuhan; Lin, Jinghao; Liu, Chen; Wu, Yaosen; Wang, Xiangyang; Xu, Jiake; Jin, Haiming.
Afiliação
  • Shi Y; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
  • Ye L; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, China.
  • Shen S; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Qian T; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
  • Pan Y; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, China.
  • Jiang Y; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Lin J; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Liu C; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Wu Y; Department of Endocrinology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.
  • Wang X; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Xu J; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Jin H; The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.
Phytother Res ; 35(10): 5694-5707, 2021 Oct.
Article em En | MEDLINE | ID: mdl-34423505
ABSTRACT
Morin is a natural compound isolated from moraceae family members and has been reported to possess a range of pharmacological activities. However, the effects of morin on bone-associated disorders and the potential mechanism remain unknown. In this study, we investigated the anti-osteoclastogenic effect of morin in vitro and the potential therapeutic effects on ovariectomy (OVX)-induced osteoporosis in vivo. In vitro, by using a bone marrow macrophage-derived osteoclast culture system, we determined that morin attenuated receptor activator of nuclear factor (NF)-κB ligand (RANKL)-induced osteoclast formation via the inhibition of the mitogen-activated protein kinase (MAPK), NF-κB and calcium pathways. In addition, the subsequent expression of nuclear factor of activated T cells c1 (NFATc1) and c-fos was significantly suppressed by morin. In addition, NFATc1 downregulation led to the reduced expression of osteoclastogenesis-related marker genes, such as V-ATPase-d2 and Integrin ß3. In vivo, results provided that morin could effectively attenuate OVX-induced bone loss in C57BL/6 mice. In conclusion, our results demonstrated that morin suppressed RANKL-induced osteoclastogenesis via the NF-κB, MAPK and calcium pathways, in addition, its function of preventing OVX-induced bone loss in vivo, which suggested that morin may be a potential therapeutic agent for postmenopausal osteoporosis treatment.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoclastos / Reabsorção Óssea Idioma: En Revista: Phytother Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoclastos / Reabsorção Óssea Idioma: En Revista: Phytother Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China