Characterization of the Trans-Epithelial Transport of Green Tea (C. sinensis) Catechin Extracts with In Vitro Inhibitory Effect against the SARS-CoV-2 Papain-like Protease Activity.
Molecules
; 26(21)2021 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-34771162
ABSTRACT
This work describes an untargeted analytical approach for the screening, identification, and characterization of the trans-epithelial transport of green tea (Camellia sinensis) catechin extracts with in vitro inhibitory effect against the SARS-CoV-2 papain-like protease (PLpro) activity. After specific catechin extraction, a chromatographic separation obtained six fractions were carried out. The fractions were assessed in vitro against the PLpro target. Fraction 5 showed the highest inhibitory activity against the SARS-CoV-2 PLpro (IC50 of 0.125 µg mL-1). The untargeted characterization revealed that (-)-epicatechin-3-gallate (ECG) was the most abundant compound in the fraction and the primary molecule absorbed by differentiated Caco-2 cells. Results indicated that fraction 5 was approximately 10 times more active than ECG (IC50 value equal to 11.62 ± 0.47 µg mL-1) to inhibit the PLpro target. Overall, our findings highlight the synergistic effects of the various components of the crude extract compared to isolated ECG.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Chá
/
Catequina
/
Proteases Semelhantes à Papaína de Coronavírus
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Molecules
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Itália