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Construction of a circRNA-miRNA-mRNA network revealed the potential mechanism of Buyang Huanwu Decoction in the treatment of cerebral ischemia.
Chen, Bowei; Yi, Jian; Xu, Yaqian; Zheng, Piao; Tang, Rongmei; Liu, Baiyan.
Afiliação
  • Chen B; The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China.
  • Yi J; The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China.
  • Xu Y; The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China.
  • Zheng P; The Second Xiangya Hospital, Central South University, Changsha, China.
  • Tang R; The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China.
  • Liu B; College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China. Electronic address: liubaiyan9657@163.com.
Biomed Pharmacother ; 145: 112445, 2022 Jan.
Article em En | MEDLINE | ID: mdl-34844103
BACKGROUND AND AIM: Buyang Huanwu Decoction (BHD) is a traditional Chinese herbal medicine that is effective for treating cerebral ischemia (CI). However, the molecular mechanisms of BHD in CI have not been fully elucidated. In this study, we integrated the circular RNA (circRNA)-microRNA (miRNA)-messenger RNA (mRNA) network of middle cerebral artery occlusion (MACO) rats treated with BHD. METHODS: SD rats were randomly divided into a control group, model group, model+BHD group (2.5, 5, 10 g/kg) and model+butylphthalide (NBP) group (54 mg/kg). The neurological functions of the rats were evaluated by a modified neurological severity scoring (mNSS) system. Pathological lesions were assessed by Nissl staining, and the effects of BHD on neurovascular unit (NVU) associated protein microtubule-associated protein 2 (MAP2), glial fibrillary acidic protein (GFAP) and von Willebrand factor (VWF) were assessed by immunohistochemistry. CeRNA and miRNA microarrays were used to establish the circRNA, miRNA, and mRNA profiles. Finally, a circRNA-miRNA-mRNA ternary transcription network was constructed. RESULTS: BHD improved the neurobehavioral test scores (P < 0.01) and the histopathological changes in ischemic brain tissue in MCAO rats. The expression of MAP2 and VWF decreased and the expression of GFAP increased in the ischemic side brain tissue of MCAO rats (P < 0.01), and treatment with BHD reversed the above changes (P < 0.01 or 0.05). We identified seven, three, and 86 significantly dysregulated circRNAs, miRNAs, and mRNAs, respectively, that were associated with the neuroprotective effects of BHD. Furthermore, bioinformatics analysis showed that these targets may exert therapeutic effects through multiple pathways, such as the VEGF and Hippo signaling pathways. Finally, we constructed a circRNA-miRNA-mRNA network. CONCLUSIONS: In brief, our study provides novel insights into ceRNA-mediated gene regulation in the progression of NVU after CI and the mechanism of action for BHD.
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Texto completo: 1 Base de dados: MEDLINE Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapêuticos e Terapias MTCI: Terapias_biologicas Assunto principal: Medicamentos de Ervas Chinesas / Isquemia Encefálica / Fármacos Neuroprotetores Tipo de estudo: Prognostic_studies Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapêuticos e Terapias MTCI: Terapias_biologicas Assunto principal: Medicamentos de Ervas Chinesas / Isquemia Encefálica / Fármacos Neuroprotetores Tipo de estudo: Prognostic_studies Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China